The NEW Biometra PCR Calculator

Thermal Cyclers

Standard Thermal Cyclers:

Biometra TProfessional Basic Thermal Cycler

Biometra TProfessional Thermal Cycler

Gradient Thermal Cyclers:

Biometra TProfessional Basic Gradient Thermal Cycler

Biometra TProfessional Gradient Thermal Cycler

Multi-Block Thermal Cyclers:

Biometra T3000 Thermal
Cycler

Personal Thermal Cyclers:

Biometra TPersonal Thermal Cycler

Robotic Thermal Cyclers:

Biometra TRobot Thermal Cycler

Laboratory Plasticware

Beta Protection

Thermal Cyclers

View our full line of Biometra Thermal Cyclers

The NEW Biometra PCR Calculator

Simplify the setup of your PCR reaction with the NEW Biometra PCR Calculator Excel Tool. You can download the full version of the PCR Calculator excel file or one of the four individual tools within the workbook.

Below are the 4 Different Types

PCR Protocol Designer
The protocol designer allows you to create PCR programs for a variety of applications like conventional PCR, fast-PCR or long-range PCR. Enter the general parameters for the protocol design as suggested by the manufacturer and target specific PCR parameters like primer annealing temperature, product length and number of cycles. From this information a suitable PCR program is created automatically. The Protocol is able to be modified using special settings for gradient function or touchdown PCR.

View the PCR Protocol Designer Calculator…

PCR Mastermix Designer
Simply enter the number of reactions, reaction volume and stock concentrations and the final PCR mastermix is calculated automatically. For documentation of the results a print out of the PCR Mastermix Designer Worksheet with an inserted gel picture can be generated.

View the PCR Mastermix Designer Calculator…

PCR Multiplex-Mix Designer
Similar to the PCR mastermix designer but in this tab mastermixes for a multiplex PCR (up to 10 different targets) can be designed. For reaction less than a 10-plex the filter function serves for a clear representation of the ‘active’ primer pairs.

View the PCR Multiplex-Mix Designer Calculator…

Primer Calculator
The primer calculator uses an algorithm (*developed by Marmur & Doty, 1962) to calculate annealing temperatures. Just enter the primer sequence and the amount of lyophilzed primer to obtain the melting temperature and a suggestion of how to prepare a stock solution. These dilutions can be calculated from OD₂₆₀ Measurements.

View the PCR Primer Calculator…

If you have questions regarding the PCR Calculator and its individual tools contact our Biometra Product Manager.

*Marmur & Doty, Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109-18.

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