LABREPCO - Biometra Thermal Cyclers & PCR Machines

The TOptical Thermal Cycler combines state of the art technology with functional design, creating one of the most flexible real-time PCR thermal cyclers. This thermal cycler is based on the reliable Biometra TProfessional 96 well thermal cycler (which can be upgraded to real-time with an additional module), providing excellent temperature uniformity and high speed due to the excellent heat conductivity of the silver block. Other new product features include:

Product Features:

Existing TProfessional can be upgraded to Real-Time (TOptical Module)
Optional gradient available
Free customization with color filter modules

- Definition of the number of filters to be implemented

- Choice of 10 different filters (6 fluorescence, 4 FRET)

- Filter combinations covering the whole range of commonly used dyes

Patented scanner system with optical fibers
Optimal excitation by three long lasting LEDs
Easy to use software including commonly used analysis functions
Superior heating and cooling (6°C/sec. and 4.5°C/sec.)

Ordering Info
Part Number	Description	Properties
070-500	TOptical Gradient 96	without color filter module
070-501	TOptical 96	without color filter module
070-510	TOptical Gradient 96 Module	without color filter module
070-511	TOptical 96 Module	without color filter module
070-520	TOptical Filter Module 1	470nm/520nm
070-521	TOptical Filter Module 2	515nm/545nm
070-523	TOptical Filter Module 3	535nm/580nm
070-524	TOptical Filter Module 4	565nm/605nm
070-525	TOptical Filter Module 5	630nm/670nm
070-526	TOptical Filter Module 6	660nm/705nm
070-527	TOptical FRET Module 1	470nm/580nm
070-528	TOptical FRET Module 2	470nm/670nm
070-529	TOptical FRET Module 3	470nm/705nm
070-530	TOptical FRET Module 4	515nm/670nm

Model	TOptical 96 (Non-Gradient)	TOptical 96 (Gradient)
Block Capacity	TOptical 96 96 Well TOptical 96 Module 96 Well	TOptical 96 Gradient 96 Well TOptical 96 Gradient Module 96 Well
Lid Temperature	30-99º C	30-99º C
Temperature Gradient	N/A	40º C
Max. Heating Rate (Avg. Heating Rate)	6.0º C/sec (5.0º C/sec)	6.0º C/sec (5.0º C/sec)
Max. Cooling Rate (Avg. Cooling Rate)	4.5º C/sec (3.5º C/sec)	4.5º C/sec (3.5º C/sec)
Ramp Adjusment	min. 0.1°C / sec, max. 5.0°C / sec	min. 0.1°C / sec, max. 5.0°C / sec
Block Temperature Uniformity (15 sec after clock starts)	± 0,15°C at 55°C ± 0,25°C at 72°C ± 0,50°C at 95°C	± 0,15°C at 55°C ± 0,25°C at 72°C ± 0,50°C at 95°C
Control Accuracy	± 0.10°C	± 0.10°C
Temperature Range Block	3 to 99º C	3 to 99º C
Temperature Increments	min. 0,1°C/cycle	min. 0,1°C/cycle
Heated Lid	manual opening mechanism, automatic pressure application	manual opening mechanism, automatic pressure application
Contact Pressure Heated Lid	10 kg, automated	10 kg, automated
Control Mode	remote controlled via PC	remote controlled via PC
No. of Programs	unlimited on PC	unlimited PC
Dimensions	28cm x 38cm x 43cm 28cm x 64cm x 43cm when opened	28cm x 38cm x 43cm 28cm x 64cm x 43cm when opened
Weight	15.6 kg (TOptical module 7.4 kg, TProfessional base unit 8.2) kg)	15.6 kg (TOptical module 7.4 kg, TProfessional base unit 8.2) kg)
Max Power Consumption	Base unit max. 480W TOptical module max. 25W	Base unit max. 480W TOptical module max. 25W
Power Supply	100V, 110V, 230V	100V, 110V, 230V
Operating Conditions	15°C to 35°C, max. 70% humidity, max. 2000 m height	15°C to 35°C, max. 70% humidity, max. 2000 m height
Supporting Plastic Ware	96 well microplates with adhesive optical foil, strips of 8, 0.2ml with optical lids, 0.2 ml single tubes with optical lids, 0.2 ml single tubes with optical lids	96 well microplates with adhesive optical foil, strips of 8, 0.2ml with optical lids, 0.2 ml single tubes with optical lids, 0.2 ml single tubes with optical lids
Sensitivity	1 nmol / I FAM at 30 µl sample volume in a 96 well PCR plate	1 nmol / I FAM at 30 µl sample volume in a 96 well PCR plate
Measuring Time	96 well plate (single measurement, 6 colors) appr. 6 sec)	96 well plate (single measurement, 6 colors) appr. 6 sec)
Measuring Range	± 130 000 (±17 bit)	± 130 000 (±17 bit)
Sample Volume	5-80 µl	5-80 µl
Light Source	Three long living high power LEDs (blue, white, red)	Three long living high power LEDs (blue, white, red)
Lightpath	An array of 8 high performance optical fibers in a shuttle system directs LED light bundled by lenses to samples. The fluorescent dyes in the reaction mix are excited through from above through the lid of the tubes. The reflected light is focused by lenses and directed through optical fibers to a photomultiplier	An array of 8 high performance optical fibers in a shuttle system directs LED light bundled by lenses to samples. The fluorescent dyes in the reaction mix are excited through from above through the lid of the tubes. The reflected light is focused by lenses and directed through optical fibers to a photomultiplier
Detector	Highly sensitive channel photo multiplier (CPM), Optimal signal/noise ration by effective noise reduction (decreased SNR [signal/noise ratio]-technology)	Highly sensitive channel photo multiplier (CPM), Optimal signal/noise ration by effective noise reduction (decreased SNR [signal/noise ratio]-technology)
Color Modules	6 color modules for all commonly used real-time PCR dyes, 4 FRET filter combinations	6 color modules for all commonly used real-time PCR dyes, 4 FRET filter combinations
qPCRsoft	Control and analysis software	Control and analysis software
Analysis Methods	Absolute quantification Relative quantification ΔΔCt method Allelic discrimination Efficiency calculation	Absolute quantification Relative quantification ΔΔCt method Allelic discrimination Efficiency calculation
Export Functions	Excel, REST, qBASE, Biogazelle	Excel, REST, qBASE, Biogazelle
Security	Administrator function MIQE-compliant sample documentation	Administrator function MIQE-compliant sample documentation

Filter

Biometra offers six different color filter modules and four FRET filter modules for the TOptical Thermal cycler. The six filter modules cover a spectrum from green to far red emission. For each filter, examples of dyes that can be detected are given in the table below. Of course, other dyes having similar excitation and emission maxima may be used. In addition, for FRET applications, four filter modules are available. The corresponding dye combinations are given in the table below.

Filter	Wavelengths (abs./em.)	Dyes
TOptical Filter Module 1	470nm/520nm	FAM, SYBR Green, Alexa488
TOptical Filter Module 2	515nm/545nm	JOE, VIC, HEX, Yakima Yellow
TOptical Filter Module 3	535nm/580nm	TAMRA, DFO, Alexa 546, NED
TOptical Filter Module 4	565nm/605nm	ROX, TexasRed, Cy3.5
TOptical Filter Module 5	630nm/670nm	Cy5, Alexa 633, Quasar 670
TOptical Filter Module 6	660nm/705nm	LightCycler Red 705, Alexa 680
TOptical FRET Module 1	470nm/580nm	FAM/TAMRA
TOptical FRET Module 2	470nm/670nm	FAM/Cy5
TOptical FRET Module 3	470nm/705nm	FAM/Cy5.5
TOptical FRET Module 4	515nm/670nm	JOE/Cy5

TOptical Lightpath

Fluorescent light for the excitation of dyes is emitted by the long lasting LEDs. The light is transmitted by high performance optical fibers to collimator lenses. The fluorescent light is bundled and transmitted to an excitation filter of a color filter module located on a rotating filter wheel. The light is deflected by a beam splitter and transferred by optical fibers to a lens array in a shuttle that scans the sample block column by column. The light excites the fluorescent dyes in the reaction mix. The fluorescent dyes then emit light of a higher wavelength that is collected by the lenses in the shuttle system and transferred by optical fibers back to the filter color module. In the color module the light passes the beam splitter and two emission filters and is then further transferred to the channel photo multiplier for detection. Figure of the patented optical system is below.

TOptical Excitation by LED technology

The excitation spectra of the blue, white and red long lasting LED (dotted lines) cover very well the absorption spectra of the commonly used fluorescent dyes FAM, JOE/HEX/VIC, 6-ROX, TET, TAMRA, CY5 and Cy 5.5 (solid lines). In combination with the beam transfer by high perfomance optical fibers and the detection by the Channel Photo Multiplier (CPM) superb sensitivity is achieved. Excitation spectra of TOptical LEDs and absorption of fluorescent dyes can be seen in the figure below.

PCR Calculator

Simplify the setup of new PCR reactions with a NEW PCR Calculator

Product Literature

Learn more about the TOptical Thermal Cyclers by reading the product literature

User Manual

Learn how to properly use the TOptical Thermal Cyclers by reading the user manual

101 Witmer Road, Suite 700
Horsham, PA 19044
P: 1-800-521-0754
F: 215-442-9202 sales@labrepco.com

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