TOptical 96 Thermal Cycler

TOptical 96
(Non-Gradient)

96 Well

30-99º C

N/A

Max. Heating Rate
(Avg. Heating Rate)

6.0º C/sec (5.0º C/sec)

Max. Cooling Rate
(Avg. Cooling Rate)

4.5º C/sec (3.5º C/sec)

min. 0.1°C / sec,
max. 5.0°C / sec

± 0,15°C at 55°C
± 0,25°C at 72°C
± 0,50°C at 95°C

± 0.10°C

3 to 99º C

min. 0,1°C/cycle

manual opening mechanism, automatic pressure application

10 kg, automated

remote controlled via PC

unlimited on PC

28cm x 38cm x 43cm
28cm x 64cm x 43cm when opened

15.6 kg (TOptical module 7.4 kg, TProfessional base unit 8.2 kg)

Base unit max. 480W

100V, 110V, 230V

15°C to 35°C, max. 70% humidity, max. 2000 m height

96 well microplates with adhesive optical foil, strips of 8, 0.2ml with optical lids, 0.2 ml single tubes with optical lids, 0.2 ml single tubes with optical lids

1 nmol / I FAM at 30 µl sample volume in a 96 well PCR plate

96 well plate (single measurement, 6 colors) appr. 6 sec)

± 130 000 (±17 bit)

5-80 µl

Three long living high power LEDs (blue, white, red)

An array of 8 high performance optical fibers in a shuttle system directs LED light bundled by lenses to samples. The fluorescent dyes in the reaction mix are excited through from above through the lid of the tubes. The reflected light is focused by lenses and directed through optical fibers to a photomultiplier

Highly sensitive channel photo multiplier (CPM), Optimal signal/noise ration by effective noise reduction (decreased SNR [signal/noise ratio]-technology)

6 color modules for all commonly used real-time PCR dyes, 4 FRET filter combinations

Control and analysis software

Absolute quantification, Relative quantification, ΔΔCt method, Allelic discrimination, Efficiency calculation

Excel, REST, qBASE, Biogazelle

Administrator function
MIQE-compliant sample documentation